柏业贸易

特点及优势

特异性
GoldHotStart Taq DNA聚合酶可最大程度减少反应前（零循环）的非特异性扩增产物的产生。随着PCR温度的升高，激活的HotStart Taq聚合酶会准确扩增正确的靶序列
灵敏度
最大程度地减少了非特异性反应，出色的灵敏度和扩增效率可以检测痕量样品
便利性
每个PCR管均包含客户所需的DNA聚合酶以及PCR反应所需的所有成分，只需添加DNA模板和D.W.即可立即进行PCR反应。此外，由于包含了电泳所需的示踪染料和沉淀剂，因此易于使用，无需添加sample loading buffer
稳定性
在PCR反应混合物中加有稳定剂并冻干，即使在长时间保存后也能维持稳定的酶活性
兼容性
兼容各种样本类型。如gDNA模板、低拷贝靶标等
再现性
ISO 9001质量体系下的批量生产可最大程度地减少批次之间的差异，即使在重复实验中也可得到可重复的实验结果

应用

• High specificity PCR

• High sensitivity PCR

• gDNA template PCR

• Low-copy target PCR

• Multiple primer pairs PCR

• cDNA template PCR

• TA cloning

实验数据

Figure. Comparison of PCR amplification efficiency between AccuPower® GoldHotStart Taq PCR PreMix from Bioneer and other supplier’s Hotstart PCR master mix.
The cycling conditions for AccuPower® GoldHotStart Taq PCR PreMix were 95°C for 5 min, 30 cycles of 95°C for 30 sec, 55°C for 30 sec and 72°C for 30 sec. PCR reactions using other supplier’s PCR master mix were performed according to each supplier's protocol.
Target : human insulin receptor gene.

Lane M; 100 bp DNA Ladder(Cat. No. D-1030, Bioneer)
Lane 1; 10 ng of human genomic DNA
Lane 2; 1 ng of human genomic DNA
Lane 3; 100 pg of human genomic DNA
Lane 4; 10 pg of human genomic DNA

▶ Specifications

Enzyme	GoldHotStart Taq polymerase
5' to 3' exonuclease activity	Yes
3' to 5' exonuclease activity	No
3'- A overhang	Yes
PCR product size	~ 5 kb (human)

▶ Experimental data

Figure 1. Comparison of PCR amplification specificity between AccuPower^®GoldHotStart Taq PCR PreMix from Bioneer and other suppliers’ Hot start PCR master mix.
The cycling conditions for AccuPower^®GoldHotStart Taq PCR PreMix were 95°C for 5 min, 35 cycles of 95°C for 30 sec, 57°C for 30 sec and 72°C for 30 sec. PCR reactions using other suppliers’ PCR master mix were performed according to each supplier's protocol.
Target : ApoE gene (The PCR product size is 268 bp).

Lane M; 100 bp DNA Ladder (Cat. No. D-1030, Bioneer)
Lane 1,2; 100 ng human genomic DNA
Lane 3,4; 10 ng human genomic DNA

Figure 2. Comparison of PCR amplification specificity between AccuPower^®GoldHotStart Taq PCR PreMix from Bioneer and other suppliers’ Hotstart PCR master mix.
The cycling conditions for AccuPower^®GoldHotStart Taq PCR PreMix were 95°C for 5 min, 35 cycles of 95°C for 30 sec, 65°C for 30 sec and 72°C for 30 sec. PCR reactions using other suppliers’ PCR master mix were performed according to each supplier's protocol.

Lane M; 100 bp DNA Ladder (Cat. No. D-1020, Bioneer)
Lane 1; 100 ng human genomic DNA
Lane 2; 10 ng human genomic DNA