仪器简介
Bioneer 运用分子生物学产品研发的丰富经验，结合最新的科技成果，研制出的五通道Exicycler™实时定量PCR仪。该产品在2004年被韩国产业资源部评为同类最佳质量产品之一。仪器由热循环反应器和Bioneer独创的实时荧光信号检测模块组成，利用荧光共振能量转移原理，可以检测两种分子间的相互作用。具有高灵敏度、高准确性和系统实时整合等特性，该机器性能稳定，带梯度功能，试剂开放。拥有CE体外诊断认证，KFDA 医疗器械注册证。

仪器特点
●  五通道，带五个激发滤光片，五个检测滤光片

● 专利光路技术，使用平面光源保证均匀的照明，孔间光照强度差异最小化，无需 ROX 染料校正

● 16 bit 高灵敏度 2D CCD 检测，可一次性获得 96 孔中全部荧光信号，孔间无时间差

● 带梯度计算和温度梯度功能，便于PCR 反应条件优化

● Touch down PCR 技术（包括Time increment、Temperature increment功能）

● 波长范围 ( 490-670 nm )，支持常用的所有荧光染料和探针

● 动力学线性范围10个数量级 (10¹ - 10¹⁰)

● 系统软件界面友好，直观，易于学习和操作

● 试剂开放，耗材经济

● 认证——CE-IVD，KFDA医疗器械注册证

仪器优势
出色的2 - Dimensinal激发光源

● 采用专利的光路技术，使用平面光源保证均匀的照明，无边缘效应
● 孔间光照强度差异最小化
● 无需使用内参染料校正

增强的光学模块和检测系统

● 使用高灵敏度2 D CCD检测，可一次性获得96孔中全部荧光信号
● 孔间无时间差
● 超高压短弧光灯的明亮白光为所有的颜色提供均一的光照强度
● 光路模块可将所有孔内光损失降至最低
● 更长的灯泡使用寿命

可靠的Ct曲线

● 在没有使用内参染料校正的条件下，重复的样本间可以获得极高质量的Ct曲线

五通道技术

● 无需为内参染料保留一个通道，所有的五色通道都是可用的。

应用范围
● 基因表达定量
● 病原体检测
● 微阵列分析
● 细菌计数/病毒滴度以及鉴定反应产物
● 基于解链温度的基因型检测

实验结果
先进的光学系统允许样品的浓度信号超出系统的动态、灵敏、线形的检测范围。

                        
图1.使用Accupower^® GreenStar™ PCR预混合物（Bioneer货号：K-5040）获得的线性动态曲线和灵敏定量结果。（A和B）将目的DNA样品10倍系列稀释后，分别扩增出100 bp的产物。(C) C(T)值介于3.2-3.4，理论值为3.3。(D)标准曲线结果显示相关系数超过0.999。

                          
图2.重复性。在不同的孔中扩增出150 bp的相同目的DNA片段，设置8个平行实验。

极易操控、灵活直观的操作软件
Exicycler™操作系统以图形界面（GUI）为基础，极易学习、掌握、操作，且功能强大。并行的结果分析功能支持在实验进行中同时进行独立的操作和数据分析。该软件系统提供持续升级，能适应不同的操作和特征分析。

               
图3.Exicycler™软件的使用

Exicycler™实时定量PCR仪技术参数
 

系统参数
尺寸(mm)	540(长) * 355(宽) * 470(高)
重量	30 kg
样本容量	96 孔 / 0.2 ml管
样本体积	20 ~ 50 μl ( 50 μl recommended )
电源指标	110 V/240 V, 50/60 Hz, 850 W
环境温度	15℃-30℃
环境湿度	20%-80%
热学模块参数
温控方式	半导体( Peltier )
温度范围	4℃ - 99.9℃
温度精度	±0.3℃
温度均一性	±0.3℃
变温速率	2.5℃/sec
梯度功能	1℃-20℃ within 40℃-75℃
温度/时间增量范围	0.1℃-9.9℃ / 1sec-60 sec
光学模块参数
光源,	超高压短弧光灯( 120 W )
灯泡时长	3,000 小时
探测器	16 位 2D CCD
激发光滤片/检测光滤片	各5个
计算机参数
操作系统	Windows XP
处理速度	Intel Dual Core E2160 (1.8 GHz ) 或更高
内存	1 GB 或更高
数据接口	USB 2.0 或更高
显示器分辨率	1280*1024或更高

   

产品技术咨询
Email: ascn@bioneer.com


证书



使用Exicycler™ 96发表的文章
  

Ref.#	Reference
0018	Gene expression profiling: Canonical molecular changes and clinicopathological features in sporadic colorectal cancers Jin Cheon Kim, Seon Young Kim, Seon Ae Roh, Dong-Hyung Cho, Dae Dong Kim, Jeong Hyun Kim and Yong Sung Kim. World J Gastroenterol. 2008 Nov 21;14(43):6662-6672. More information
0017	Ultra-rapid real-time PCR for the detection of Paenibacillus larvae, the causative agent of American Foulbrood (AFB) Sang-Hoon Han, Do-Bu Lee, Dong-Woo Lee, Eul-Hwan Kim and Byoung-Su Yoon. J Invertebr Pathol. 2008 Sep;99(1):8-13. Epub 2008 May 6. More information
0016	CpG methylation in exon 1 of transcription factor 4 increases with age in normal gastric mucosa and is associated with gene silencing in intestinal-type gastric cancers Seung-Kyoon Kim, Hay-Ran Jang, Jeong-Hwan Kim, Mirang Kim, Seung-Moo Noh, Kyu-Sang Song, Gyeong Hoon Kang, Hee Jin Kim, Seon-Young Kim, Hyang-Sook Yoo and Yong Sung Kim.. Carcinogenesis. 2008 Aug;29(8):1623-1631. Epub 2008 Jul 16. More information
0015	Two homologous parasitism-specific proteins encoded in Cotesia plutellae bracovirus and their expression profiles in parasitized Plutella xylostella Sunyoung Lee and Yonggyun Kim. Arch Insect Biochem Physiol. 2008 Apr;67(4):157-171. More information
0014	A viral lectin encoded in Cotesia plutellae bracovirus and its immunosuppressive effect on host hemocytes Sunyoung Lee, Madanagopal Nalini, Yonggyun Kim. Comp Biochem Physiol A Mol Integr Physiol. 2008 Apr;149(4):351-361. Epub 2008 Jan 14. More information
0013	Dissection of the essential steps for condensin accumulation at kinetochores and rDNAs during fission yeast mitosis Norihiko Nakazawa, Takahiro Nakamura, Aya Kokubu, Masahiro Ebe, Koji Nagao and Mitsuhoro Yanagida. J Cell Biol. 2008 Mar 24; 180(6):1115-1131. More information
0012	Epigenetic inactivation of protein kinase D1 in gastric cancer and its role in gastric cancer cell migration and invasion Mirang Kim, Hay-Ran Jang, Jeong-Hwan Kim, Seung-Moo Noh, Kyu-Sang Song, June-Sik Cho, Hyun-Yong Jeong, Jim C. Norman, Patrick T. Caswell, Gyeong Hoon Kang, Seon-Young Kim, Hyang-Sook Yoo and Yong Sung Kim. Carcinogenesis. 2008 Mar;29(3):629-637. Epub 2008 Feb 17. More information
0011	Eicosanoids mediate prophenoloxidase release from oenocytoids in the beet armyworm Spodoptera exigua Sony Shrestha and Yonggyun Kim. Insect Biochem Mol Biol. 2008 Jan;38(1):99-112. Epub 2007 Oct 6. More information
0010	Differential expression profile of genes encoded in a genome segment of Cotesia plutellae bracovirus in a parasitized host, Plutella xylostella Wael GAD, Jae Young Choi, Yeon Ho Je and Yonggyun Kim. Entom Res. 2008; 38:77-86.
0009	Protein tyrosine phosphatases encoded in Cotesia plutellae bracovirus: sequence analysis, expression profile, and a possible biological role in host immunosuppression Ahmed M.A. Ibrahim, Jae Young Choi, Yeon Ho Je and Yonggyun Kim. Dev Comp Immunol. 2007;31(10):978-990. Epub 2006 Dec 13. More information
0008	Testicular expression of steroidogenic enzyme genes is related to a transient increase in serum 19-nortestosterone during neonatal development in pigs Nag-Jin Choi, Jin Hee Hyun, Jae Min Choi, Eun Ju Lee, Kyung Hyun Cho, Yunje Kim, Jongsoo Chang, Il Byung Chung, Chung Soo Chung and Inho Choi. Asian-Aust J Anim Sci. 2007 Dec;20(12):1832-1842.
0007	A novel culture technique for human embryonic stem cells using porous membranes Sinae Kim, Seong Eun Ahn, Jae Ho Lee, Do-Seon Lim, Kwang-Soo Kim, Hyung-Min Chung and Soo-Hong Lee. Stem Cells. 2007 Oct;25(10):2601-2609. Epub 2007 Jul 12. More information
0006	Effect of aging on insulin secretory function and expression of beta cell function-related genes of islets Sung-Hee Ihm, Hong Ju Moon, Jun Goo Kang, Cheol Young Park, Ki Won Oh, In Kyung Jeong, Yang-Seok Oh, Sung Woo Park. Diabetes Res Clin Pract. 2007 Sep;77 Suppl 1:S150-154. Epub 2007 Apr 30. More information
0005	The real-time polymerase chain reaction Mikael Kubista, Jose Manuel Andrade, Martin Bengtsson, Amin Forootan, Jiri Jonak, Kristina Lind, Radek Sindelka, Robert Sjoback, Bjorn Sjogreen, Linda Strombom, Anders Stahlberg and Neven Zoric. Mol Aspec Medi. 2006;27:95-125.
0004	Identification of genes related to Parkinson's disease using expressed sequence tags Jeong-Min Kim, Kyu-Hwa Lee, Yeo-Jin Jeon, Jung-Hwa Oh, So-Young Jeong, In-Sung Song, Jin-Man Kim, Dong-Seok Lee and Nam-Soon Kim. DNA Res. 2006 Dec 31;13(6):275-286. Epub 2007 Jan 8. More information
0003	Antioxidants ameliorate the expression of vascular endothelial growth factor mediated by protein kinase C in diabetic podocytes Eun-Young Lee, Choon Hee Chung, Jung Hyun Kim, Hea-Jung Joung and Sea Yong Hong. Nephrol Dial Transplant. 2006 Jun;21(6):1496-1503. Epub 2006 Feb 16. More information
0002	AtRTPrimer: database for Arabidopsis genome-wide homogeneous and specific RT-PCR primer-pairs Sangjo Han and Dangsup Kim. BMC Bioinformatics. 2006 Mar 30;7:179. More information
0001	GLR1 plays an essential role in the homeodynamics of glutathione and the regulation of H2S production during respiratory oscillation of Saccharomyces cerevisiae Ho-Yong Sohn, Eun-Joo Kum, Gi-Seok Kwon, Ingnyol Jin, Claire A. Adams and Hiroshi Kuriyama. Biosci Biothchnol Biochem. 2005 Dec; 69(12):2450-2454. More information

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