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荧光定量PCR
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  • 商品名称: Exicycler™ 96 荧光定量PCR仪
  • 属性释义: Superior 5-color Real-Time Quantitative PCR system

询价

 

 

Bioneer 运用分子生物学产品研发的丰富经验,结合最新的科技成果,研制出Exicycler®实时定量PCR仪。该产品在2004年被韩国产业资源部评为同类最佳质量产品之一。仪器由热循环反应器和Bioneer独创的实时荧光信号检测模块组成,利用荧光共振能量转移原理,可以检测两种分子间的相互作用。由于具有高灵敏度、高准确性和系统实时整合等特性,使之成为高通量分析仪器的最佳选择。

 

 

主要应用

  病毒载量测定
  病原体检测
  癌症研究
  遗传疾病检测
  耐药分析
  DNA甲基化研究
  SNP检测
  基因表达研究

 

主要特性及优势

 

 检测五种荧光团的多通路技术
 极高的灵敏度和广泛的动态检测范围
 易于系统维护的自我恢复功能
 可同时检测96个样品的荧光信号
 高解析度和有效的熔解曲线分析
 宽范围的激发光和发射光波长
 提供均匀和同步的温度梯度
 提供实验中实时数据分析
 优良的兼容性
 光照均匀,降低孔间差别

 

 

产品优势

 LT technology(专利光导管技术)

BIONEER采用专利的光导管技术,确保平板中每一个孔内光照的均匀和采集到的信号的准确性,从而无需使用内参染料(Rox dye等)对实验结果进行校准和修正
 

 精准的Ct值重复性

在无内参染料校正的条件下,选择热模块的不同位置(如下图),设置9个重复的样本进行实验,可以获得重复性极高的ct值(数据差异低于0.5)
 

 真正的5重荧光PCR技术

因为无需使用内参染料对实验结果进行校准,所以所有5个荧光检测通道都可以用于靶核酸序列的检测。并且激发光源配备对应的5组激发滤光片和检测滤光片,保证不同波长荧光之间的干扰降至最低,保证多重PCR实验结果的准确性。
 

 

 高灵敏度的光学检测器

高灵敏度的16-bit 2D CCD检测器可一次性采集96孔中的全部荧光信号,无检测时间差。相比较光电倍增管(PMT)检测器,无需使用移动的机械臂,降低仪器的机械故障。
 

 出色的激发光源

为了保证实验结果的准确性,高能量的激发光源是最主要的指标之一。光源的质量是荧光激发效果的决定性因素之一。Exicycler96采用超高压短弧灯做为荧光的激发光源,具有能量高寿命长的特点,是荧光定量PCR仪的理想光源。


  系统自检功能

仪器操作软件具有系统自检功能,用户可以方便地对仪器硬件进行检查,排除实验故障和维护。
 

 样品仓自动开关功能

仪器的样品仓具有自动开关的功能,方便用户对实验室设备的自动化升级。例如为仪器增配自动加样系统。
 

 温度梯度及Touchdown功能

热模块具有温度梯度功能,可设置12道梯度温度,方便实验条件的优化。可设置时间温度增量,实现Touchdown功能。
 

                                  

 

 友好的用户界面
模块化操作软件非常简单易学, 即使是刚接触荧光定量PCR仪的使用者也可以迅速掌握实验设计和数据分析。

 

实验应用

 

 

 参考文献

 

Ref.#

Reference

20

Functional Switching of TGF-β1 Signaling in Liver Cancer via Epigenetic Modulation of a Single CpG Site in TTP Promoter
Bo Hwa Sohn, etc. Korea Research Institute of Bioscience and Biotechnology
Gastroenterology Volume 138, Issue 5, May 2010

19

Dissection of the essential steps for condensin accumulation at kinetochores and rDNAs during fission yeast mitosis
Norihiko Nakazawa, etc. Core Research for Evolutional Science and Technology Research Program,
Kyoto University
J Cell Biol. 2008 Mar 24; 180(6):1115-1131.

18

Nucleotide change of codon 182 in the surface gene of hepatitis B virus genotype C leading to truncated surface protein is associated with progression of liver diseases
Seoung-Ae Lee, etc.
College of Medicine, Seoul National University
Journal of Hepatology Aug., 2011

17

Corticotropin-releasing hormone downregulates IL-10 production by adaptive forkhead box protein 3–negative regulatory T cells in patients with atopic dermatitis
Sang Ho Oh, etc.
Yonsei University College of Medicine
Journal of Allergy and Clinical Immunology Sep., 2011

16

Human ZNF312b Promotes the Progression of Gastric Cancer by Transcriptional Activation of the K-ras Gene
In-Sung Song, etc. Genome Research Center, Korea Research Institute of Bioscience and Biotechnology
Cancer Research.  Apr., 2009

15

Functional and Clinical Evidence for NDRG2 as a Candidate Suppressor of Liver Cancer Metastasis
Dong Chul Lee, etc. Medical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology
Cancer Research.  Jun., 2008

14

LRRC3B, Encoding a Leucine-Rich Repeat-Containing Protein, Is a Putative Tumor Suppressor Gene in Gastric Cance
Mirang Kim, etc. Medical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology
Cancer Research.  Sep., 2008

13

Chondrogenic potential of stem cells derived from amniotic fluid, adipose tissue, or bone marrow encapsulated in fibrin gels containing TGF-β3
SJi Sun Parka, etc.
College of Life Science, CHA University

Biomaterials  Nov., 2011

12

Multi-lineage differentiation of hMSCs encapsulated in thermo-reversible hydrogel using a co-culture system with differentiated cells
Ji Sun Parka, etc.
College of Life Science, CHA University
Biomaterials  Volume 31, Issue 28, Oct., 2010

11

Determination of dual delivery for stem cell differentiation using dexamethasone and TGF-β3 in/on polymeric microspheres
SJi Sun Parka, etc.
College of Life Science, CHA University

Biomaterials  Sep., 2009

10

Zap70 Functions to Maintain Stemness of Mouse Embryonic Stem Cells by Negatively Regulating Jak1/Stat3/c-Myc Signaling
Young Cha, etc.
College of Life Science, CHA University
STEM CELLS  Volume 28, Issue 9, pages 1476–1486, Sep., 2010

09

A novel culture technique for human embryonic stem cells using porous membranes

Sinae Kim, etc. CHA Stem Cell Institute, Pochon CHA University

STEM CELLS  2007 Oct;25(10):2601-2609

08

Human Cord Blood-Derived Endothelial Progenitor Cells and Their Conditioned Media Exhibit Therapeutic Equivalence for Diabetic Wound Healing
H Ji Yeon Kim, etc.
College of Life Science, CHA University
Cell Transplantation  Volume 19, Number 12, 2010

07

CpG methylation in exon 1 of transcription factor 4 increases with age in normal gastric mucosa and is associated with gene silencing in intestinal-type gastric cancers

Seung-Kyoon Kim, etc. Medical Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology

Carcinogenesis  2008 Aug;29(8):1623-1631. Epub 2008 Jul 16.

06

Sprouty1 Regulates Neural and Endothelial Differentiation of Mouse Embryonic Stem Cells

Jee-Eun Jung, etc. Department of Biomedical Science, College of Life Science, CHA University,

Stem Cells and Development  May, 2011

05

Galanin antagonist increases insulin resistance by reducing glucose transporter 4 effect in adipocytes of rats

Lili Guo, etc. 扬州大学医学院

General and Comparative Endocrinology  August, 2011 Pages 159-163

04

Heparin attenuates lipopolysaccharide-induced acute lung injury by inhibiting nitric oxide synthase and TGF-β/Smad signaling pathway

En Mu, etc. 中国医科大学第一附属医院

Thrombosis Research  October, 2011

03

Elevated Expression of Liver X Receptor Alpha (LXRα) in Myocardium of Streptozotocin-Induced Diabetic Rats

Yongxia Cheng, etc. 中国医科大学

Inflammation  December, 2010

02

Reduced expression of Toll-like receptor 4 inhibits human breast cancer cells proliferation and inflammatory cytokines secretion

Huan Yang, etc. 苏州大学附属第二医院

Journal of Experimental & Clinical Cancer Research  July, 2010

01

Up-regulated biglycan expression correlates with the malignancy in human colorectal cancers

Xiaohu Gu, etc. 辽宁省肿瘤医院

Clinical and Experimental Medicine  August, 2011

*以上为使用Bioneer实时荧光定量PCR仪发表的部分文献,更多文献请见各学术期刊。

Manual

Exicycler™ 96

 

Brochure

Exicycler™ 96

 

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001:2008 - certificate

• EN ISO 13485: 2003 - certificate

• EC Directive 98/79/EC - certificate

 

Technical support

Email: ascn@bioneer.com

 

认证

 

Cat. No.

Product Description

Price

Add to Cart

A-2060

Exicycler™ 96 Real-Time Quantitative Thermal block

询价

 

关键词: 定量PCR仪 荧光定量

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