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  • 商品名称: miRNA模拟物&抑制剂

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AccuTarget™ Human miRNA mimics & inhibitors

Bioneer's AccuTarget™ miRNA mimics are chemically synthesized, double-stranded RNA oligonucleotidess and available for 2,042 Human Mature microRNAs in the miRBase Sequence Database. AccuTarget™ miRNA inhibitors are the single-stranded synthetic inhibitor targeting all human miRNAs in the miRBase Sequence Database. These miRNA mimics & inhibitors are available at 5, 10 and 20 nmole guaranteed yield. We also offer miRNA mimics and inhibitors library sets consisting of predesigned mimics or inhibitors at various small scales (0.25, 0.5, 1, or 2 nmole) in a 96-well plate layout to meet the unique needs of individual customers. . In addition, flexible miRNA library sets for customer- specified mimics & inhibitors are also available for the minimum order of 48 ea.


 

Features and Benefits

Ready-to-transfect miRNA mimics behave like endogenous miRNAs and inhibitors suppress target miRNA activity to study loss-of-function effects after transfection into cells

 


 Purification: For your more demanding applications, Bioneer's automated HPLC and Bio-RP purification methods ensure high quality, high-throughput miRNA mimics and inhibitors at an affordable price.
 Affordable pricing: Bioneer provides a variety of high quality miRNA products at an affordable price.
 Synthesis and QC: Bioneer miRNA mimics and inhibitors are produced in clean room facility by fully automated high-throughput miRNA production system. Bioneer miRNA products are assessed by MALDI-TOF Mass spectrometry analysis. Mass spec data is provided with every miRNA mimic and inhibitor. Additionally, miRNA mimics are tested by gel electrophoresis to verify that both RNA strands annealed properly.


All Bioneer miRNA inhibitors are provided as single-stranded miRNA* (antisense strand of target miRNA) and all Bioneer miRNA mimics are provided as double-stranded siRNA. Each sense siRNA and an antisense siRNA are QC'ed by MALDI-TOF analysis. Every annealed double-stranded miRNA is then QC-tested using PAGE to confirm proper annealing.





 

AccuTarget™ miRNA mimic controls

We offer AccuTarget™ miRNA mimic controls to optimize assay conditions for miRNA mimic function studies. Both positive and negative controls are provided for miRNA gain-of-function studies using Bioneer's AccuTarget™ miRNA mimics.

AccuTarget™ miRNA housekeeping Positive controls target the 3' UTR (untranslated region) of the standard housekeeping gene, GAPDH, and BIONEER's miRNA mimic Negative controls' sequences are based on common miRNA structure for use as negative experimental controls in human, mouse, and rat cells. The negative controls have been analyzed by BLAST against all human, mouse and rat genomic sequences and miRNA sequences in the current miRBase Database. BIONEER offers two universal negative controls for mimics. In addition, AccuTarget™ miRNA control sets consisting of a Positive and two Negative miRNA controls are also available for user convenience.


 

Features and Benefits

 

 Excellent performance: miRNA Houskeeping Positive controls targeting GAPDH with clear read-out of mimic function (knockdown efficiency of >90%) miRNA mimic Negative controls with minimal sequence identity with miRNAs in human, mouse and rat.
 Monitoring of transfection rate: fluorescently labeled Negative controls for conveniently monitoring cellular uptake and/or transfection efficiency
 Competitive pricing: Great value for your research dollar

Introduction

MicroRNA

MicroRNAs (miRNAs) are 21-25 nucleotide(nt)-long single-stranded RNA molecules that serve as a post-transcriptional regulator of gene expression in eukaryotes. The human genome may encode over 1000 miRNAs, which bind with imperfect complementarity to their target mRNAs, generally within the 3’UTR (untranslated region), and repress protein production by destabilizing the mRNA as well as translational suppression. miRNA-mediated translational repression has important roles in wide range of biological process, including development, cell proliferation and differentiation, apoptosis and metabolism [1].
 

MicroRNA Pathway

The biogenesis of miRNAs consists of two sequential processing events. Primary miRNA transcripts (pri-miRNAs), which contain one or multiple stem-loop hairpin structures, are mostly derived from Pol II-mediated transcription. In first step towards the canonical miRNA maturation pathway, pri-miRNA is cleaved by the microprocessor complex, RNase Ⅲ enzyme Drosha, to yield the pre-miRNA, a hairpin-shaped intermediate precursor ~70 nt in length. Pre-miRNAs are then exported from the nucleus to the cytoplasm by Exprotin5, where another RNaseⅢ enzyme Dicer catalyzes the second processing event for miRNA biogenensis and liberates the mature miRNA duplexes. The mature miRNA duplexes consist of the mature miRNA strand and the miRNA* strand, which are derived from two separate arms of the hairpin stem within the miRNA precursor. The miRNA is loaded into an Argonaute-containing RNA-induced silencing complex (RISC), whereas the miRNA* strand is typically degraded. The Ago:miRNA complex then dissociates from RISC loading complex, and become the core of the RISC complex to regulate post-transcriptional gene repression of specific target mRNAs[2].

 

 

A recently identified extensive class of small RNAs, called miRNA, has provided new insights in biotechnology. Although they were discovered and recognized relatively recently, miRNAs have been recognized as the most important gene regulators at the post-transcriptional level, and several studies indicated that miRNAs regulate the expression of more than 30 % protein coding genes [3]. The accumulating knowledge about their biogenesis, gene expression regulation mechanism and functions will add a new dimension to our understanding about the complex gene regulatory networks. Recent investigations demonstrate that miRNAs have a unique expression profiles in different cancer types at different stages and play an important role in many disease and viral infections. These results suggest that miRNAs can function as a novel biomarker for disease diagnosis and perform a new strategy for miRNA gene therapy [4].

 

1. Sci china Ser C-Life Sci, 2009, 52(4):323-330
2. Annu. Rev. Cel. Dev. Biol. 2007. 23:175-205
3. Virchows Arch. 2008. 452:1-10
4. J. Cell. Mol. Med. 2008. 12(1): 3-21

Performance of miRNA mimic Positive and Negative controls

Figure1 . AccuTarget™ miRNA Positive & Negative controls were transfected at 20 nM using Lipofectamine™ RNAiMAX into HeLa cell lines and assessed for their ability to decrease target mRNA levels. Down-regulation of GAPDH was determined using the real-time quantitative RT-PCR at 48 hours post-transfection using Bioneer's Exicylcer™ 96 qPCR instrument.

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