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Broad RNAi Portfolio from Bioneer

Over 132,000 predesigned siRNA's, also validated and standard siRNA libraries by gene function or family - AccuTarget

 

Bioneer的核心业务是在常规制造方法上加以技术性地突破生产及销售DNARNA。从发现了RNA干扰(RNAi)之后开始,Bioneer的研究团队研发了各种实验目的所需的高品质siRNA产品。Bioneer目前不仅是亚洲RNAi技术的领导者,siRNA产品还远销北美地区高等学府及研究单位。BioneerRNAi产品主要有以下6部分组成。


• Predesigned siRNA libraries
• PCR primers for knockdown validation
• Human validated siRNA libraries
• Human siRNA library sets and subsets
• Control siRNAs
• siRNA design and synthesis



Bioneer的所有siRNA都是利用本公司与韩国国家基因信息中心合作共同研发出的一种siRNA选择淘汰专利系统(Turbo si-Designer设计的。Turbo si-Designer软件通过现有研究排除已知的nonfunctionalsiRNA site,考虑到多种因素(base composition, thermodynamic instability, base preference 为客户寻找具有极高沉默效率的最优化的siRNA target site。另外,设计时避免了目前已知的SNP位点,通过BLASTSmith-Waterman运算筛查清除了non-specific siRNABioneer提供的所有siRNA产品在突出的沉默效率及最优化的off-target effect都得到了光大客户的认可。客户购买3AccuTarget siRNA后如果其中2条沉默效率在80%以下,Bioneer将免费提供两条siRNA 

 

  • The Bioneer 80% Guarantee

    购买同一基因的3siRNA,Bioneer保证其中至少2siRNA的沉默效率不低于80%,如有两条沉默效率小于80%,Bioneer将免费提供2siRNA

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    *投诉必读:投诉时请提供如下2supporting data

    1. siRNA knockdown efficiency data: NC (AccuTarget Negative Control) and siRNA concentration at 100 nM

    2. Transfection efficiency data: PC (AccuTarget GAPDH/GFP/Luciferase siRNA) and NC (AccuTarget Fluorescein-labeled Negative Control)

     

    Introduction

    Until recently, gene knockdown or knockout technologies, such as antisense, ribozyme, and gene knockouts were used to perform loss-of-function studies. However, the post-genomics era calls for high-throughput gene function studies which the former technologies were unable to answer due to poor reproducibility, high cost, and excessive time to results. The advent of siRNA technology has opened up many new possibilities in the field of gene suppression.
     

    siRNA Mechanism

    siRNA is the term for 20 - 25-base pair RNA duplexes, where the two terminal 3'-nucleotides are unpaired (3' overhang). When siRNAs are introduced into cells they combine with a protein complex called the RNA-induced silencing complex (RISC) and are unwound by a helicase. The RISC complex containing single stranded RNA complementary to the target mRNA then recognizes and binds to the target mRNA. After binding the mRNA, the argonaute protein Ago2 cleaves it and complete degradation of the target mRNA is carried out by ribonuclease activity (as a result of the lack of protection by 5' caps or poly (A) tails). This exciting technology is one of the most effective methods for the silencing of specific target genes and is a must for gene function validation studies, drug target validation, and for gene therapy studies. siRNA has the following advantages over other RNAi technology:

    • Reduced time and costs: Less screening is needed to obtain highly effective siRNA.
    • High efficacy at lower concentration: lower concentrations provide effective gene silencing and minimizes off target effects
    • Specificity: siRNA is a highly specific target knockout mechanism based on the natural biological mechanisms of RNAi



    sirna mechanism

    Bioneer manufactures high quality and cost-effective siRNA. Every siRNA is produced in an automated high-throughput RNA production system under clean room conditions and undergo rigorous QC tests.


    Features and Benefits

    Performance Guarantee: When purchasing 3 siRNAs for the same gene, Bioneer guarantees at least an 80% reduction* in the target mRNA level for 2 of the three siRNAs.
    Purification: For your more demanding applications, Bioneer's automated HPLC and Bio-RP purification methods ensure high quality, high- throughput siRNAs at an affordable price.
    Affordable pricing: Bioneer provides a variety of high quality siRNA products at an affordable price.
    Synthesis and QC: Bioneer siRNAs are produced in clean room facility by fully automated high-throughput siRNA production system. Bioneer siRNA products are assessed by MALDI-TOF Mass spectrometry analysis. Mass spec data is provided with every siRNA. Additionally, siRNAs are tested by gel electrophoresis to verify that both RNA strands annealed properly.

    All Bioneer siRNAs are provided as double-stranded siRNA. Each sense siRNA and an antisense RNA are QC'ed by MALDI-TOF. Every annealed siRNA is then QC-tested using PAGE to confirm proper annealing.

    rnai figure2

    Figure 1. MALDI-TOF mass spectrometry analysis of a custom siRNA. All siRNAs are processed by MALDI-TOF mass spectrometry to ensure its quality.


    rnai figure3

    Figure 2. PAGE data of double-stranded custom siRNA. Complementary single-stranded RNA strands were hybridized to form siRNA duplex and analyzed by 15% non-denaturing PAGE.
    SS: single-stranded RNA
    DS: double-stranded siRNA


    rnai figure4

    Figure 3. Confocal microscopic image of HeLa cells transfected with FITC-labeled negative control siRNA (Cat No.: SN-1021). The fluorescent cells indicate that the HeLa cells were successfully transfected with the siRNA.


    rnai figure5

    Figure 4. Effects of Human GAPDH Positive Control siRNA. HeLa cells were transfected separately with AccuTarget Human GAPDH Positive Control and Negative Control siRNA using Lipofectamine 2000 (Invitrogen) at a final concentration of 100 nM. Total cellular RNA was isolated from cells 24 hours after transfection and subjected to Northern blot and Real-Time PCR analysis. As can be seen from Fig. 1-B, about 3% GAPDH mRNA remained.


    Contact

    Bioneer, Inc.
    Phone: 1-877-264-4300
    To place an order: order.usa@bioneer.us.com
    Email: support@bioneer.us.com


    Notice to Purchaser

    All siRNA Products: For Research Use Only. Not For Use in Diagnostic Procedures.


    Limited License

    This product is licensed under European Patents 1144623, 121945 and foreign equivalents from Alnylam Pharmaceuticals, Inc., Cambridge, USA and is provided only for use in academic and commercial research whose purpose is to elucidate gene function, including research to validate potential gene products and pathways for drug discovery and development and to screen non-siRNA based compounds (but excluding the evaluation or characterization of this product as the potential basis for a siRNA-based drug) and not for any other commercial purposes. Information about licenses for commercial use (including discovery and development of siRNA-based drugs) is available from Alnylam Pharmaceuticals, Inc., 300 Third Street, Cambridge, MA 02142, USA.

    This product is sold for research use only and is not to be administered to humans or used for medical diagnostics. Buyer acknowledges and agrees that all intellectual property rights in the products (including, without limitation, the siRNA sequences used to create such products) and in any Bioneer technology, intellectual property and know-how used to make or useful for the manufacture or use of the products will at all times remain vested in Bioneer (other than any ownership interest that buyer may have in non-public proprietary target genes supplied by buyer to Bioneer in connection with custom products).

    Trademark: AccuTarget is a trademark of Bioneer Corporation.

    Synthesis and Order


    Q1. siRNA是如何合成的?

     

    目前最普遍的siRNA合成方法是用β-cyanoethyl phosphoramidite把形成RNA构造骨架的phosphodiester连接起来的'phosphite triester'法。 (Nucl. Acids Res. 1984, 12, 4539 ; Tetrahedron Lett. 1983, 24,5843)整个合成过程是从nucleoside附着的CPG状态开始,经过deblocking, coupling, capping, oxidation反复循环过程得到所需长度的siRNA。合成结束后通过ammonia处理的siRNACPG上脱离,再经过deprotection过程和TBDMS处理过程然后纯化得到高纯度siRNA。合成的单链RNA通过退火过程最终得到双链siRNA

    Q2.如何订购 Pre-design siRNA?

     

    我公司销售的AccuTarget Genome-wide predesigned siRNA产品包括了Human, Mouse, Rat在内全部的预设计siRNA. 可以通过siRNA网站 (http://sirna.bioneer.com)输入Symbol, Gene No, Refseq accession No, Description等信息查看对于各个genepredicted efficiency score,您可以发送邮件到sales@bioneercn.com,把您所需条数、纯化方式、终产量发给我们下单。

    Q3. 从下单到收到产品需要多长时间?

    预设计siRNA从下单到上海需要5~7个工作日,客户定制siRNA到上海需要7~9个工作日,另外加修饰通常会多1~3个工作日。

    *因为所有产品均在韩国生产,所以要经过上海子公司统一清关发货,上面所述时间为韩国从生产到上海接收货物时间。

    Q4.Bioneer最多可以合成到多少个碱基?

     

    Single strand RNA最多50mersiRNA最多30mer。如果要合成30mer以上的siRNA请直接联系我们技术部门。邮箱:ascn@bioneer.com 电话:021-50801191-802

    Q5. 客户收到的siRNA是什么型态的?

     

    Genome-wide predesigned siRNA, Validation siRNA, siRNA Library, Control siRNA是把SenseAntisense纯化成统一浓度(MALDI-TOF mass 检测)后进行退火形成双链siRNA(冻干粉形式)提供给客户。客户收到产品直接用buffer或者附赠的DEPC D.W.溶解到所需浓度(建议100μM)使用即可。Custom siRNA直接用buffer或者附赠的DEPC D.W.溶解到所需浓度(建议50μM)使用即可。Custom siRNA下单时请注意是否勾选退火,如果没有要求退火的话,需要用annealing buffer(1X)以及附带的annealing protocol自行退火。

     

    更多常见问题请点击这里


     RNAi 以前年度论文检索 



    2013年 2012 2011 2010



     2014年论文列表 
     

    Product Reference
    RNAi Development of biocompatible apatite nanorod-based drug delivery system with in situfluorescence imaging capacity
    Rajendra Singh, Tae-Hyun Kim, Kapil Patel, Jung-JuKim, Hae-Won Kim
    Journal of Materials Chemistry B,DOI: 10.1039/C3TB21156H
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    RNAi HDAC2 provides a critical support to malignantprogression of hepatocellular carcinoma throughfeedback control of mTORC1 and AKT
    Ji Heon Noh, Hyun Jin Bae, Jung Woo Eun, qingyuShen, Se JIn Park, Hyung Seok Kim, Boas Nam,Woo Chan Shin, Eun Kyung Lee, Kyoung Bun Lee,Ja-June Jang, Won Sang Park, Jung Young Lee,and Suk Woo Nam
    Cancer Research, doi:10.1158/0008-5472.CAN-13-2109
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    RNAi AUF1 contributes to Cryptochrome1 mRNAdegradation and rhythmic translation
    Kyung-Ha Lee, Sung-Hoon Kim, Hyo-Jin Kim,Wanil Kim, Hwa-Rim Lee, Youngseob Jung, Jung-Hyun Choi, Ka Young Hong, Sung Key Jang andKyong-Tai Kim
    Nucleic Acids Research, Nucl.Acids Res. (2014)doi: 10.1093/nar/gkt1379Nucl.Acids Res. (2014)doi: 10.1093/nar/gkt1379
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    RNAi Autophagy Regulates Homeostasis ofPluripotency-Associated Proteins in hESCs
    Yun-Hee Cho, Kyu-Min Han, Dongkyu Kim,Joonsun Lee, Sang-Hee Lee, Kyeng-Won Choi,Jungho Kim, Yong-Mahn Han
    STEM CELLS, Volume 32, Issue 2,pages 424–435, February 2014
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    RNAi Combined delivery of HMGB-1 box A peptide andS1PLyase siRNA in animal models of acute lunginjury
    Binna Oh, Minhyung Lee
    Journal of Controlled Release,Volume 175, 10 February 2014,Pages 25–35
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    RNAi Akt phosphorylates and activates HSF-1independent of heat shock, leading to Slugoverexpression and epithelial–mesenchymaltransition (EMT) of HER2-overexpressing breastcancer cells
    R L Carpenter, I Paw, M W Dewhirst and H-W Lo
    Oncogene, doi:10.1038/onc.2013.582
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    RNAi Selenoprotein S is a marker but not a regulator ofendoplasmic reticulum stress in intestinal epithelialcells
    Bodo Speckmanna, Kirsten Gerloffb, c, LisaSimmsd, Iulia Oanceab, c, Wei Shia, Michael A.McGuckinb, c, Graham Radford-Smithd, e, f, KumKum Khanna
    Free Radical Biology andMedicine, Volume 67, February2014, Pages 265–277
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    RNAi Polymer nanoparticles for enhanced immune response: Combination delivery of tumor antigen and siRNA for immunosuppressive gene to dendritic cells
    Min Beom Heo, Mi Young Cho, Yong Taik Lim
    Acta Biomaterialia, doi:10.1016/j.actbio.2013.12.050
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    RNAi Multifunctional hybrid nanocarrier: Magnetic CNTs ensheathed with mesoporous silica for drug delivery and imaging system
    Rajendra Kumar Singh, Kapil D. Patel, Jung-Ju Kim, Tae-Hyun Kim, Joong-Hyun Kim, Ueon Sang Shin, Eun-Jung Lee, Jonathan C. Knowles, and Hae-Won Kim
    acs Applied materials & interfaces, DOI:10.1021/am4056936
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    RNAi The RNA-binding Protein HuD Regulates Autophagosome Formation in Pancreatic β Cells by Promoting Autophagy-related Gene 5 Expression
    Chongtae Kim, Wook Kim, Heejin Lee, Eunbyul Ji, Yun-Jeong Choe, Jennifer L. Martindale, Wado Akamatsu, Hideyuki Okano, Ho-Shik Kim, Suk Woo Nam, Myriam Gorospe, Eun Kyung Lee
    Journal of Biological Chemistry, The Journal of Biological Chemistry, 289, 112-121.
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    RNAi Human Kruppel-related 3 (HKR3) is a novel transcription activator of alternate open reading frame (ARF) gene
    Jae-Hyeon Yoon1, Won-Il Choi1, Bu-Nam Jeon1, Dong-In Koh1, Min-Kyeong Kim1, Myung-Hwa Kim1, Jungho Kim2, Sujin Susanne Hur3, Kyung-Sup Kim1 and Man-Wook Hur1
    J BIOL CHEM, doi:10.1074/jbc.M113.526855
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    RNAi Down-regulation of Mortalin Exacerbates Aβ-mediated Mitochondrial Fragmentation and Dysfunction
    So Jung Park, Ji Hyun Shin, Jae In Jeong, Ji Hoon Song, Yoon Kyung Jo, Eun Sung Kim, Eunjoo H. Lee, Jung Jin Hwang, Eun Kyung Lee, Sun Ju Chung, Jae-Young Koh, Dong-Gyu Jo, and Dong-Hyung Cho
    Journal of Biological Chemistry, The Journal of Biological Chemistry, 289, 2195-2204.
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    RNAi Sphingosine-1-phosphate-induced Flk-1 transactivation stimulates mouse embryonic stem cell proliferation through S1P1/S1P3-dependent β-arrestin/c-Src pathways
    Jung Min Ryu, Young Bin Baek, Myung Sun Shin, Ji Hoon Park, Soo Hyun Park, Jang Hern Lee, Ho Jae Han
    Stem Cell Research, Volume 12, Issue 1, January 2014, Pages 69-85
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    RNAi The GLI1 splice variant TGLI1 promotes glioblastoma angiogenesis and growth
    Hu Zhua, Richard L. Carpenter, Woody Han, Hui-Wen Lo
    Cancer Letters, Volume 343, Issue1, 1 February 2014, Pages 51–61
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    RNAi Highly improved specificity for hybridization-based microRNA detection by controlled surface dissociation
    Hye Ryeon Yoon,ab Jeong Min Lee,c Juyeon Jung,ab Chang-Soo Lee,ab Bong Hyun Chung*ab and Yongwon Jung
    Analyst, 2014,139, 259-265
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    RNAi NSC126188 induces apoptosis of prostate cancerPC-3 cells through inhibition of Akt membranetranslocation, FoxO3a activation, and RhoBtranscription
    Kyoung-Jae Won, Bo Kyung Kim, Gyoonhee Han,Kyeong Lee, Young-Jin Jung, Hwan-Mook Kim,Kyung Bin Song, Kyung-Sook Chung, Misun Won
    Apoptosis, January 2014, Volume19, Issue 1, pp 179-190
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    RNAi Effect of salts, solvents and buffer on miRNAdetection using DNA silver nanocluster(DNA/AgNCs) probes
    Pratik Shah1, Seok Keun Cho1, Peter WaabenThulstrup2, Yong-Joo Bhang3, Jong Cheol Ahn3,Suk Won Choi3, Andreas Rørvig-Lund2,4 andSeong Wook Yang
    Nanotechnology, Nanotechnology25 (2014) 045101 (7pp)
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    Dong Hee Kim, Han Rae Kim, Eun Jung Choi,Dong Yeol Kim, Kwang Kon Kim, Byung Sam Kim,Jeong Woo Park, Byung Ju Lee
    PLOS ONE, DOI:10.1371/journal.pone.0085898
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    PLOS ONE, DOI:10.1371/journal.pone.0085546
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    american journal of physiology-renal physiology, Vol. 306no.F155-F167DOI:10.1152/ajprenal.00438.2013
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    FEBS letters, Volume 588, Issue 1,3 January 2014, Pages 79-85
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    Park E, Kang H, Kim J, Ko J
    Cellular physiology andbiochemistry,DOI:10.1159/000356658
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    Eun Jung Choi, Bong Jun Cho, David J Lee, YeoHyeon Hwang, Sun Ha Chun, Hans H Kim and InAh Kim
    BMC Cancer, BMC Cancer 2014,14:17 doi:10.1186/1471-2407-14-17
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    Hye Rin Lee1,†, Hwa Kyoung Shin2, So Youn Park1,Hye Young Kim1, Won Suk Lee1,3, Byung YongRhim3, Ki Whan Hong1, Chi Dae Kim1,3,*
    Journal of Neuroscience Research,Volume 92, Issue 2, pages 206-217, February 2014
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    Journal of Ethnopharmacology,doi: 10.1016/j.jep.2013.11.055
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    Archives of DermatologicalResearch,
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    Jinmi Lee, Seok-Woo Hong, Se Eun Park, Eun-JungRhee, Cheol-Young Park, Ki-Won Oh, Sung-WooPark, Won-Young Lee
    CELL STRESS CHAPERON,10.1007/s12192-013-0490-3
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    Bo Hyung Lee, Pan Dong Ryu and So Yeong Lee
    International Journal of MolecularSciences, Int. J. Mol. Sci. 2014, 15,977-993;doi:10.3390/ijms15010977
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    So Young Eun, Jihye Seo, Sang Won Park, JaeHeun Lee, Ki Churl Chang, Hye Jung Kim
    InternationalImmunopharmacology, Volume18, Issue 2, February 2014, Pages270-276
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    Biochemical and BiophysicalResearch Communications,Volume 444, Issue 1, 31 January2014, Pages 63–68
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    Mi-Kyoung Kima, Hyun-Joo Parka, b, Yong-DeokKimc, Mi Heon Ryud, Takashi Takatae, Soo-yungBaeb, Moon-Kyoung Baea,
    Archives of Oral Biology, Volume59, Issue 2, February 2014, Pages102-110
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